According to Chargaff's rule, which pair of nitrogenous bases aligns together?

Chargaff's rule states that in the DNA of any given organism, the amounts of adenine (A) are equal to thymine (T), and the amounts of guanine (G) are equal to cytosine (C). This pairing is a fundamental aspect of the DNA double helix structure, where adenine pairs specifically with thymine through two hydrogen bonds, establishing a key aspect of base pairing that ensures the stability of the DNA molecule.

In the context of Chargaff's findings, adenine and thymine consistently align together due to their complementary structures, allowing for efficient pairing that supports proper DNA replication and integrity. The presence of these specific pairings also facilitates the accurate transmission of genetic information during cell division, making this pairing crucial for biological processes.